Mycoplasmas in Tissue Culture

Mycoplasmas in Tissue Culture

Mycoplasmas are frequently found as contaminants in tissue-cultured cells. Infections may be inapparent or cause severe cytopathic changes. The source of primary contaminations in most cases is probably the upper respiratory tract of man. The wide dissemination of infections in cultures most probably occurs as a result of aerosols set up during the processing of contaminated cultures. Mycoplasm...

Contamination of Tissue Cultures by Mycoplasmas

Mycoplasmas are the smallest and simplest self-replicating bacteria [1]. These microorganisms lack a peptidoglycan based rigid cell wall and thus are not susceptible to antibiotics, such as penicillin and its analogues, which are effective against most bacterial contaminants of cell cultures. The trivial name mycoplasma encompasses all species included in the class Mollicutes: i.e. the genera M...

Survival of Bordetella pertussis in Tissue Culture of Animal Cells

Tissue Culture

Dr. Willmer's second edition of this little book fulfills his stated purpose ... to correlate some of the numerous fragments of scattered data which have so far been obtained and to orientate them in such a way as to illustrate the more fundamental contributions which the method has made to physiological knowledge, and to show the manner in which a change of outlook has already been brought abo...

Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique

Mycoplasma gallisepticum and Mycoplasma synoviae are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tools, polymerase chain reaction (PCR) and culture are confirmatory tools for the diagnosis of mycoplasmosis after the initial serological screening of suspected birds. Overall, 181 samples were analyzed, 152 (84%) and 103 (57%) of which were found positive...